Pig skin microbiota Metagenome. skin metagenome

We investigated the effect of scabies on skin microbial communities in a porcine model. In a 21 week trial, 96 skin scrapings from 15 pigs were collected before and during infection and after acaricide treatment and analysed by pyrosequencing of bacteria 16S rDNA (V1-V3). We found significant changes in the epidermal microbiota, in particular a dramatic increase in Staphylococcus correlating with the onset of mite infestation, which persisted beyond treatment and healing. Furthermore, the staphylococci population shifted from the commensal S. hominis on the healthy skin prior to scabies mite challenge to S. chromogenes, which is increasingly recognized as being pathogenic, coinciding with scabies infection in pigs. In contrast, all animals in the scabies-free cohort remained relatively free of Staphylococcus throughout the trial. This is the first in vivo study directly monitoring scabies mite infection associated disruption of the healthy microbiota and supporting previous assumptions that scabies promotes the establishment of pathogens.

本研究以猪为模型，探究了疥疮对皮肤微生物群落的影响。在为期21周的试验中，研究人员采集了15头猪的96份皮肤刮拭样本，采集节点涵盖感染前、感染期间以及杀螨剂治疗后，并通过细菌16S rDNA（V1-V3区）焦磷酸测序对样本进行分析。研究发现表皮微生物群发生显著变化，其中葡萄球菌属（Staphylococcus）的丰度随疥螨侵染发作出现急剧升高，且该变化在治疗与愈合后仍持续存在。此外，随着猪疥疮感染的发生，葡萄球菌种群从疥螨侵染前健康皮肤上的共生人葡萄球菌（S. hominis）转变为如今逐渐被认定为致病菌的猪葡萄球菌（S. chromogenes）。与之形成对照的是，全程未感染疥疮的对照组猪只始终未检测到明显的葡萄球菌属定植。本研究是首个直接监测疥螨感染引发健康微生物群失调的体内研究，同时验证了此前关于疥疮可促进病原体定植的相关假说。