Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi ChIP-seq]. Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi ChIP-seq]

Polycomb repressive complexes (PRC) 1 and 2 are essential chromatin regulators of cell identity. PRC1, a dominant executer of Polycomb-mediated control, functions as multiple sub-complexes that possess catalytic-dependent H2AK119 mono-ubiquitination (H2AK119ub) and catalytic-independent activities. Here, we show that despite its well-established repressor functions, PRC1 binds to both silent and active genes. Through in vivo loss-of-function studies, we show that global PRC1 function is essential for skin development and stem cell (SC) specification. Overall design: Newborn mouse epidermal progenitor cells were FACS purified. Immunoprecipitated chromatin was analyzed by ChIP-seq.

多梳蛋白抑制复合体1与2（Polycomb repressive complexes, PRC）是维持细胞身份所必需的核心染色质调控因子。作为多梳介导基因调控的主要执行因子，PRC1以多种亚复合体形式存在，兼具催化依赖型组蛋白H2A第119位赖氨酸单泛素化（H2AK119ub）活性与催化非依赖性功能。本研究表明，尽管PRC1的转录抑制功能已被广泛证实，但其既可结合沉默基因，也可结合活跃基因。通过体内功能缺失实验，我们证实全局PRC1功能对于皮肤发育与干细胞（SC）特化至关重要。实验整体设计：通过荧光激活细胞分选（FACS）纯化新生小鼠表皮祖细胞，对免疫沉淀获得的染色质进行染色质免疫共沉淀测序（ChIP-seq）分析。