ChIP-seq analysis reveals alteration of H3K4 trimethylation occupancy of cancer-related genes by cold atmospheric plasma. ChIP-seq analysis reveals alteration of H3K4 trimethylation occupancy of cancer-related genes by cold atmospheric plasma

Cold atmospheric plasma (CAP) has obtained credits for cancer treatment by its ability to preferentially induce cancer cell death, while its molecular mechanism is under elucidation. Here, an epigenetic effect of CAP on cancer cell was examined by performing a genome-wide ChIP-seq for H3K4me3 in the MCF-7 breast cancer cell. Overall design: an epigenetic effect of CAP on cancer cell was examined by performing a genome-wide ChIP-seq for H3K4me3 in the MCF-7 breast cancer cell.

冷大气等离子体（Cold Atmospheric Plasma, CAP）因其可选择性诱导癌细胞死亡的特性，在癌症治疗领域获得了认可，但其分子机制仍有待阐明。本研究通过对MCF-7乳腺癌细胞中的组蛋白H3赖氨酸4三甲基化（H3K4me3）开展全基因组染色质免疫共沉淀测序（ChIP-seq），探究了冷大气等离子体对癌细胞的表观遗传效应。实验整体设计：通过对MCF-7乳腺癌细胞中的H3K4me3进行全基因组染色质免疫共沉淀测序（ChIP-seq），分析冷大气等离子体对癌细胞的表观遗传效应。