Microbiome analysis of biofilms electrodes from MES Metagenome. biocathode metagenome

The present study aims at gaining knowledge on how the start-up process influences the microbial communities that develop on the biofilm of biocathodes. This is done by assessing the impact of the microbial diversity of the inoculum and the starting potential of the bioelectrode. For this purpose, we tested a highly diverse inoculum such as river mud (RM), and a more specialised one such as anaerobic digestate (AD). We also evaluated the impact of the starting potential by either operating the working electrode as an anode and then switching it to cathode, or directly operating the working electrode as a cathode. This approach resulted in four different start-up strategies, and for all of them, we provide an analysis of the evolution of microbial communities together with information of the reactor performance (in terms of current production and product formation).

本研究旨在解析启动过程对生物阴极（biocathodes）生物膜上定殖微生物群落的影响机制。为此，我们通过考察接种物的微生物多样性与生物电极的初始电位的作用效果开展相关研究。具体而言，我们选用了两类接种物：一类是微生物多样性极高的河泥（RM），另一类是特异性更强的厌氧消化液（AD）。此外，我们还通过两种方式调控工作电极的初始电位：先将工作电极作为阳极运行，随后切换为阴极；或直接将工作电极设置为阴极运行。该实验方案共形成四种不同的启动策略，针对所有策略，我们均对微生物群落的演替过程进行了分析，并同步提供了反应器的运行性能数据（以电流产生与产物生成为评价指标）。