Transcriptome Profiling Identified Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids. Transcriptome Profiling Identified Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids

The aim of the present study was to explore commonly and preferentially expressed genes for male and female gamete development in synthetic Brassica allohexaploids. We performed differential expression analysis to identify precise genes that regulate developmental events during male and female gamete development of Brassica allohexaploids. Overall design: The leaf, anther from meiosis to pollen maturation and ovule from meiosis to mature embryo sac formation of synthetic Brassica allohexaploids have three repetitions respectively, and the leaves are the control group.

本研究旨在探究人工合成芸苔属（Brassica）异源六倍体雌雄配子发育过程中普遍表达与偏好性表达的基因。本研究通过差异表达分析，鉴定出调控芸苔属异源六倍体雌雄配子发育进程的精准功能基因。整体实验设计：人工合成芸苔属异源六倍体的叶片、减数分裂至花粉成熟阶段的花药，以及减数分裂至成熟胚囊形成阶段的胚珠，均设置三次生物学重复，其中叶片作为对照组。