EZ cleared tissues mounting strategies and visual assessment of clearing quality

Figure 1: EZ cleared tissues mounting strategies and visual assessment of clearing quality. A: Mouse spinal cord mounted in a custom tissue holder, with pins securing the tissue in place. B: Mouse brain attached to a nut using glue. C: mouse brain cleared with EZ clear protocol on a graph millimeter grid. D: perfused and post-fixed mouse brain (uncleared) E: bad clearing outcome due to THF stock solution contamination with oxygen and peroxide formation. F: inhomogeneous clearing of mouse brain tissue G: good clearing of muse brain on a text page with font size 11.

图1：EZ透明化组织的挂载策略与透明化质量目视评估。A：小鼠脊髓置于定制组织支架中，以固定针将组织固定就位。B：使用粘合剂将小鼠大脑粘贴至螺母上。C：采用EZ clear方案完成透明化的小鼠大脑，置于毫米方格网格纸上。D：经灌注及后固定的未透明化小鼠大脑。E：因四氢呋喃（THF）原液被氧气污染并生成过氧化物，导致透明化效果不佳。F：小鼠脑组织透明化不均匀。G：透明化效果优良的小鼠大脑，置于标注11号字体的文本页面上。