Identification of Hepatic Genes Regulated by ATF3 using Next Generation Sequencing

This study aimed to investigate how many genes were regulated by hepatocyte ATF3. C57BL/6J mice were injected intravenously with adeno-associated virus (AAV) expressing human activating transcription factor 3 (ATF3) under the control of an albumin promoter (AAV8-ALB-ATF3) or AAV8-ALB-Null). After 8 weeks, liver samples were used for RNA sequencing using Illumina NextSeq 500. Over 3000 genes were found to be up- or down-regulated by ATF3. Overall design: Ten weeks old C57BL/6J mice were intravenoulsy injected with AAV8-ALB-ATF3 or AAV8-ALB-Null (control) (n=8 per group). After 8 weeks, total RNA was extracted and cDNA libraries were prepared. RNA sequencing was carried out using Next-Seq 500. Data were analyzed using Illumina Softwares in BaseSpace.

本研究旨在探究肝细胞激活转录因子3（activating transcription factor 3, ATF3）所调控的基因数量。将C57BL/6J小鼠经静脉注射携带人源激活转录因子3（ATF3）的腺相关病毒（adeno-associated virus, AAV），该病毒以白蛋白启动子（albumin promoter）调控ATF3的表达（AAV8-ALB-ATF3），或注射AAV8-ALB-Null作为对照。8周后采集肝脏样本，使用Illumina NextSeq 500平台进行RNA测序。研究发现超过3000个基因受ATF3调控，出现表达上调或下调。
实验整体设计：选取10周龄的C57BL/6J小鼠，经静脉注射AAV8-ALB-ATF3或AAV8-ALB-Null（对照组，每组n=8）。8周后提取总RNA并构建cDNA文库，使用NextSeq 500平台开展RNA测序，数据通过BaseSpace中的Illumina软件进行分析。