Hordeum vulgare subsp. vulgare cultivar:Barke Transcriptome or Gene expression. Hordeum vulgare subsp. vulgare cultivar:Barke

Transcriptome profiling of endosperm subdomains from barley grains at the beginning of endosperm celluzlarization (3 to 4 days after flowering). RNA-seq of transfer cells (ETCs) and the remaining syncytium (Sync) was performed to compare developmental and regulatory programs triggering cell specification in the early endosperm. Subdomains/cells were isolated by laser capture microdissection (LCM), mRNA was prepared to generate sequencing libraries and libraries were sequenced with HiSeq 2500 (Illumina).

本数据集针对开花后3~4天、胚乳细胞化起始阶段的大麦籽粒胚乳亚结构域开展转录组分析（Transcriptome profiling）。研究对传递细胞（ETCs，transfer cells）与剩余合胞体（Sync，syncytium）进行RNA测序（RNA-seq），以对比早期胚乳中触发细胞特化的发育与调控程序。实验通过激光捕获显微切割（LCM，laser capture microdissection）分离亚结构域与细胞，提取信使RNA（mRNA）以构建测序文库，并使用HiSeq 2500（Illumina）完成文库测序。