Localization of antibodies to BAI1, Noggin and MyoD in the skin, eyes and brain.

Human lens tissue and tissue sections of human tattooed skin, rabbit anterior cavity, rat retina and mouse brain were double labeled with the G8 and R&D BAI1 mAbs and antibodies to Noggin and MyoD. The total numbers of double labeled cells were counted in a subset of tissue sections. The total numbers of single labeled cells were quantified in all sections with the exception of MyoD-/R&D BAI1 mAb+ and MyoD-/G8- cells that were counted in a subset of skin and anterior cavity sections. The numbers of tissues and sections scored are indicated in parenthesis. The results are the mean ± standard deviation. The R&D BAI1 mAb co-localized with G8 and Noggin with rare exception. BAI1+ cells without MyoD were present in the skin and anterior cavity.

本研究采用G8与R&D BAI1单克隆抗体（monoclonal antibody，mAb）以及靶向Noggin、MyoD的抗体，对人晶状体组织、人纹身皮肤组织切片、兔眼前腔、大鼠视网膜及小鼠脑组织进行双重标记。针对部分组织切片，统计了双重标记细胞的总数量。对所有组织切片中的单标记细胞总数进行定量分析，但MyoD⁻/R&D BAI1 mAb⁺及MyoD⁻/G8⁻细胞除外——此类细胞仅在部分皮肤与前腔组织切片中完成计数。本次分析涉及的组织与切片数量已标注于括号内。实验结果以平均值±标准差表示。R&D BAI1单克隆抗体与G8及Noggin几乎共定位，仅存在极少数例外情况。不表达MyoD的BAI1⁺细胞可在皮肤与前腔组织中被检测到。