Assessment of Endocrine Disruption in the Shenandoah River Watershed – Transcriptome Data from Mobile Laboratory Fish Exposures and Other Experiments Conducted during 2015

Here, we employed integrated chemical and biological analyses to determine how environmental mixtures affected biological responses in watersheds with different landuse. Adult male fathead minnows (Pimephales promelas) were exposed to water from different locations within the Shenandoah River watershed (VA, USA) in 2014, 2015, and 2016. The exposure locations were chosen to capture unique landuse in surrounding watersheds, including agricultural, municipal, mixed-use, and forested sites. Gene expression profiles were measured in livers of male fish exposed for 7 days using Agilent 60K custom FHM microarrays. Independent statistical analyses were conducted for each year, using a reference site (PCFH) as the comparison. Differentially expressed probes were identified by one-way ANOVA followed by FDR correction. This study identified site-specific patterns of differentially expressed genes. ---------------------------------- NFSRD - North Fork Shenandoah River above tributary near Woodstock, VA NRWD - North River below WWTP near Mount Crawford, VA NRWU - North River above WWTP near Mount Crawford, VA PCFH - Passage Creek at fish hatchery at Waterlick, VA SFSR - South Fork Shenandoah River above WWTP at Bentonville, VA

本研究采用化学生物整合分析方法，探究环境复合污染物对不同土地利用类型流域内生物响应的影响。2014至2016年间，将成年雄性黑头软口鲦（Pimephales promelas）暴露于美国弗吉尼亚州谢南多厄河流域内不同采样点位的水体中。本次选取的暴露点位覆盖了流域周边各异的土地利用类型，包括农业用地、市政用地、混合用地及林地。采用安捷伦（Agilent）60K定制化黑头软口鲦（FHM）基因芯片，对暴露7天后的雄性鱼肝组织进行基因表达谱检测。以参照点位PCFH作为对照，每年单独开展独立的统计学分析。通过单因素方差分析（one-way ANOVA）结合错误发现率（FDR）校正，筛选得到差异表达探针。本研究明确了各采样点位特异性的差异基因表达模式。
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NFSRD——弗吉尼亚州伍德斯托克附近支流上游的北岔谢南多厄河
NRWD——弗吉尼亚州克劳福德山附近污水处理厂（WWTP）下游的北河
NRWU——弗吉尼亚州克劳福德山附近污水处理厂上游的北河
PCFH——弗吉尼亚州沃特利克鱼类孵化场处的帕萨奇溪
SFSR——弗吉尼亚州本顿维尔附近污水处理厂上游的南岔谢南多厄河