DataSheet2_Complete genome sequence of a novel Prescottella sp. R16 isolate from deep-sea sediments in the western Pacific.xlsx

Prescottella, a distinct genus separate from Rhodococcus, has garnered attention for its adaptability and ecological versatility. In this study, a Gram-stain positive and ovoid-rod shaped the actinobacterium strain R16 was isolated from deep-sea sediment (with a depth of 6,310 m) in the Western Pacific. On the basis of 16S rRNA gene sequence analysis, average nucleotide identity and phylogenomic analysis, strain R16 clearly represents a novel species within the genus Prescottella. Genomic analyses indicate Prescottella sp. R16 contains a circular chromosome of 4,531,251 bp with an average GC content of 68.9%, 4,208 protein-coding genes, 51 tRNA genes, and 12 rRNA operons. Additionally, four CRISPRs and 24 genomic islands are also identified. The presence of rich categories related to catalytic activity, membrane part and metabolic process highlights their involvement in cellular component, biological process, and molecular function. The genome sequence of strain R16 also revealed the presence of 13 putative biosynthetic gene clusters for secondary metabolites, including those for ε-Poly-L-lysine, ectoine, heterobactin, isorenieratene and corynecin, suggesting its potential for antibiotic production and warranting further exploration.

普雷斯科特菌属（Prescottella）是独立于红球菌属（Rhodococcus）的独特菌属，因其良好的适应性与生态多功能性受到学界广泛关注。本研究从西太平洋水深6310米的深海沉积物中，分离得到一株革兰氏染色阳性（Gram-stain positive）、卵状杆状的放线菌菌株R16。基于16S rRNA基因序列分析、平均核苷酸同一性（average nucleotide identity）与系统发育基因组学分析，菌株R16明确隶属于普雷斯科特菌属中的一个新物种。基因组分析结果显示，菌株R16包含一条长度为4531251 bp的环状染色体，平均GC含量为68.9%，共编码4208个蛋白质编码基因、51个转运RNA（tRNA）基因以及12个核糖体RNA（rRNA）操纵子。此外，该基因组还被鉴定出4个成簇规律间隔短回文重复序列（CRISPR）与24个基因组岛。该菌株基因组中富集了大量与催化活性、膜组分及代谢过程相关的基因类别，凸显其在细胞组分构成、生物学过程执行与分子功能发挥中的关键作用。菌株R16的基因组序列还揭示其含有13个潜在的次级代谢产物生物合成基因簇，涵盖ε-聚-L-赖氨酸（ε-Poly-L-lysine）、四氢嘧啶（ectoine）、异铁菌素（heterobactin）、异红菌烯（isorenieratene）与棒杆菌素（corynecin）等类型，这表明其具备抗生素生产潜力，有待进一步深入探索。