Transcriptomic analysis of the role of the integrin a6b4 in detached cells. Transcriptomic analysis of the role of the integrin a6b4 in detached cells

Analysis of gene expression profiles of matrix-detached cells with and without expression of ITGB4, in clustering and non-clustering conditions. The experiment tested the hypothesis that the integrin beta 4 (ITGB4) mediates a significant amount of pro-survival signaling in matrix-detached conditions. Expression of ITGB4 in cancer is correlated with poor patient survival and is impliated in increased metastatic spread. Survival in matrix-deprived conditions is essential to metastasis and targeting signaling downstream of the integrin beta 4 may help curtail metasasis. Overall design: Examination of gene expression profile following 3 hours of incubation in matrix-deprived conditions in duplicate

本研究针对黏附脱离细胞在细胞聚集与非细胞聚集条件下、分别表达与不表达整合素β4（ITGB4）的基因表达谱展开分析。本实验旨在验证如下假说：整合素β4可在黏附脱离状态下介导大量促存活信号通路。肿瘤组织中ITGB4的表达与患者不良生存预后相关，且与肿瘤转移扩散程度加剧有关。黏附剥夺状态下的细胞存活是肿瘤转移的必要前提，靶向整合素β4下游信号通路或可抑制肿瘤转移。实验整体设计：将细胞置于黏附剥夺条件下孵育3小时后，对样本进行两份重复的基因表达谱分析。