A polysaccharide fraction extracted from Pleurotus ostreatus mycelial biomass inhibit Sarcoma 180 tumor

ABSTRACT Fungi of Pleurotus genus have attracted a great interest due to their medicinal properties such as anti-inflammatory, antimicrobial and antitumor. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. This work aimed to study the mycelial growth of P. ostreatus in submerged culture, evaluating the influence of the initial concentration of substrate (20 and 40 g/L of glucose) and the pH (4 and 6) on kinetic parameters of production of biomass. The effectiveness of different doses (10, 30 and 50 mg/kg) of a mycelium polysaccharide fraction extracted from P. ostreatus in reducing Sarcoma 180 development in mice was also verified. In the range of this study, maximum concentration of mycelial biomass (about 12.8 g/L) was obtained using 40.0 g/L of glucose, at pH 4.0. The total biomass productivity (Px) was not significantly affected by substrate concentration and pH, reaching values of 0.034 g/L.h. Sarcoma 180 tumor weight was reduced in 74.1, 75.5 and 53.7% when 10, 30 and 50 mg/kg were administered, respectively. These results show the high antitumor potential of intracellular polysaccharide fraction of mycelial biomass of P. ostreatus, particularly at lower doses of 10 and 30 mg/kg.

【摘要】侧耳属（Pleurotus）真菌因其抗炎、抗菌及抗肿瘤等药用特性而广受关注。此类特性主要源自侧耳属真菌合成的多糖类物质。本研究旨在探究糙皮侧耳（P. ostreatus）在液体深层培养中的菌丝生长情况，评估底物初始浓度（20与40 g/L葡萄糖）及pH值（4与6）对生物量生成动力学参数的影响。本研究同时验证了从糙皮侧耳菌丝体中提取的多糖组分以10、30、50 mg/kg三种不同剂量给药时，对小鼠肉瘤180（Sarcoma 180）生长的抑制效果。在本研究的试验条件范围内，当葡萄糖浓度为40.0 g/L、pH值为4.0时，可获得最高的菌丝生物量浓度（约12.8 g/L）。总生物量产率（Px）未受底物浓度与pH值的显著影响，其值稳定在0.034 g/L·h。分别以10、30、50 mg/kg剂量给药时，肉瘤180的肿瘤重量分别降低了74.1%、75.5%与53.7%。上述结果表明，糙皮侧耳菌丝体生物量的细胞内多糖组分具备较高的抗肿瘤潜力，尤其在10与30 mg/kg的较低剂量下效果更佳。