Coativator Function Defines the Active Estrogen Receptor Alpha Cistrome. Homo sapiens

Genome-wide ChIP-on-Chip against RNA Pol II in untreated MCF7 cells (phenol-red free media) and H3R17me2 in untreated and estrogen (45 min) treated cells. Overall design: MCF7 breast cancer cells were grown in phenol-red free media, cross-linked and processed as in (Carroll et al 2005). DNA was hybridized to GeneChip® Human Tiling 2.0R Array Set.

针对培养于无酚红培养基中的未处理MCF7细胞的RNA聚合酶II（RNA Pol II）的全基因组染色质免疫沉淀芯片（ChIP-on-Chip）分析，以及针对未处理与经雌激素处理45分钟的细胞的组蛋白H3精氨酸17位二甲基化（H3R17me2）的相关分析。实验设计方案：将MCF7乳腺癌细胞培养于无酚红培养基中，参照Carroll等（2005）的方法进行交联与样本前处理，随后将所得DNA与GeneChip®人类平铺2.0R阵列组（GeneChip® Human Tiling 2.0R Array Set）进行杂交。