Expression Profile of Circulatory Adiponectin and Plasma Variables in Broilers

ABSTRACT Adipokines serve as a human clinical biomarker and they regulate mammalian metabolic functions. Research on adipokine regulation of metabolic function in avian species is limited. The current study is to investigate the profile of plasma adiponectin and several biochemical variables in broilers, to establish the pattern of development. A total of one hundred and fifty-two 1-day-old Arbor Acres broilers were separated by different gender and homogenously divided into 2 groups (male and female), with 6 replicate pens and at least 12 chickens per pen. The data for body weight, plasma variables, and feed consumption were collected at days 3, 7, 14, 21, 28 and 35 after hatching. Plasma monomer adiponectin, high-molecular weight (HMW) adiponectin and biochemical variables, including triacylglycerol, total cholesterol, glucose, high-density lipoprotein and low-density lipoprotein were quantified. Monomer adiponectin was highly correlated with age in a negative fashion and with HMW adiponectin in a positive fashion in both genders in broilers. Moreover, body weight was highly correlated with monomer adiponectin in a negative fashion and positively correlated with HMW adiponectin in growing chickens. It was concluded that monomer adiponectin and HMW adiponectin have highly correlation with age, body weight and biochemical variables in chickens and further investigation is needed for physiological or nutritional functions.摘要 脂肪因子（adipokine）是一类人类临床生物标志物，可调控哺乳动物的代谢功能。目前针对禽类物种中脂肪因子代谢调控功能的研究仍较为有限。本研究旨在探究肉鸡血浆脂联素（adiponectin）及多项生化指标的变化特征，以明确其发育规律。本研究共纳入152只1日龄爱拔益加（Arbor Acres）肉鸡，按性别进行分组，均分为雌雄两组，每组设置6个重复饲养栏，每栏至少饲养12只肉鸡。分别于孵化后第3、7、14、21、28及35日龄采集体重、血浆指标及采食量相关数据。本研究对血浆单体脂联素（monomer adiponectin）、高分子量脂联素（high-molecular weight, HMW adiponectin）及多项生化指标进行了定量检测，包括甘油三酯（triacylglycerol）、总胆固醇（total cholesterol）、葡萄糖（glucose）、高密度脂蛋白（high-density lipoprotein）与低密度脂蛋白（low-density lipoprotein）。在两种性别的肉鸡中，单体脂联素均与年龄呈显著负相关，且与高分子量脂联素呈显著正相关。此外，生长肉鸡的体重与单体脂联素呈显著负相关，与高分子量脂联素呈显著正相关。综上，肉鸡的单体脂联素与高分子量脂联素均与年龄、体重及生化指标存在显著相关性，后续仍需开展相关研究以阐明其生理或营养调控功能。