CIL:20085

Camera lucida sketches of the zebrafish embryo at selected stages. This image is in the Segmentation period at the 12 hour 6 somite stage. The animal pole is to the top for the early stages, and anterior is to the top later, except for the two animal polar (AP) views shown below their side view counterparts for germ-ring and shield gastrulas. Face views are shown during cleavage and blastula stages. After shield stage, the views are of the embryo's left side, but before the shield arises one cannot reliably ascertain which side is which. Pigmentation is omitted. Arrowheads indicate the early appearance of some key diagnostic features at the following stages: 1 k-cell: YSL nuclei. Dome: the doming yolk syncytium. Germ ring: germ ring. Shield: embryonic shield. 75%-epiboly: Brachet's cleft. 90%-epiboly: blastoderm margin closing over the yolk plug. Bud: polster. 3-somite: third somite. 6-somite: eye primordium (upper arrow), Kupffer's vesicle (lower). 10-somite: otic placode. 21-somite: lens primordium. Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). Prim-16: heart. High-pec: pectoral fin bud. Composite image CIL: 20098 is a low resolution image with a scale bar = 250 microns that shows the entire set of image. Images correspond to Fig 1 of Developmental Dynamics 203:253-310 (1995). This material is reproduced with permission of John Wiley & Sons, Inc. Copyright 1995 WILEY-LISS, INC.

斑马鱼胚胎特定发育阶段的明视素描（Camera lucida sketch）。本图像对应12小时6体节阶段的分节期。早期胚胎视图以动物极（animal pole）为图像顶部，后期则以前端为图像顶部；仅胚环（germ-ring）与盾形期（shield）原肠胚的两张动物极（AP）视图置于其侧视图下方，属于上述规则的例外。卵裂期与囊胚期采用正面视图。盾形期之后的视图均为胚胎左侧视角，但盾形结构形成前无法可靠区分左右侧。图像未标注色素沉着细节。箭头标注了以下各阶段部分关键诊断特征的早期出现位置：1k细胞期：卵黄合胞体层（Yolk Syncytial Layer, YSL）细胞核；高顶期：隆起的卵黄合胞体；胚环期：胚环；盾形期：胚胎盾；75%外包期：布拉谢裂隙（Brachet's cleft）；90%外包期：胚层边缘闭合覆盖卵黄栓；芽基期：前突（polster）；3体节期：第三体节；6体节期：眼原基（上方箭头）与库普弗囊泡（Kupffer's vesicle，下方箭头）；10体节期：耳板；21体节期：晶状体原基；原条期6（Prim-6）：后侧线原基（背侧）与孵化腺（卵黄球表面）；原条期16（Prim-16）：心脏；高胸鳍期（High-pec）：胸鳍芽。复合图像CIL:20098为低分辨率图像，附带比例尺250微米，展示了全部图像集合。本图像对应《Developmental Dynamics》203卷第253-310页（1995年）的图1。本文本内容经John Wiley & Sons, Inc.授权转载，版权所有©1995 WILEY-LISS, INC.