Integrated omics analysis on the bacterial community in the anaerobic digestion of chicken manure materials in biogas production farm

The overall effectiveness of anaerobic digestion is affected by the initial three phases as a result of substrates created for the methanogens to take in. In this study, the bacterial composition of the various chambers in the anaerobic digestion of chicken manure was investigated. Diversity of the microbial community for chicken manure was relatively simpler, consisting mainly of Lactobacillus, compared to the main digestion chambers with a more complex community. Chicken manure only has hydrolysis phase occurring, while the main digesters have the first three phases of anaerobic digestion concurrently occurring. A comparison of the metagenomics versus metaproteomics analysis revealed that the main difference was in the percentage of unknown microorganisms. The quantity of volatile fatty acids detected in each respective chamber suggested the effectiveness of the microorganisms in assimilating the metabolites. Successful integration of these study modes would present a better understanding on anaerobic digestion of complex systems.

厌氧消化（anaerobic digestion）的整体效能受初始三个阶段影响，这些阶段会生成供产甲烷菌（methanogens）摄取的底物。本研究针对鸡粪厌氧消化过程中不同反应仓室的细菌群落组成展开了调研。相较于群落结构更为复杂的主反应仓室，鸡粪厌氧消化体系的微生物群落多样性相对较低，其群落主要由乳杆菌属（Lactobacillus）构成。鸡粪厌氧消化仅存在水解阶段（hydrolysis phase），而主消化反应器则同时发生厌氧消化的初始三个阶段。宏基因组学（metagenomics）与宏蛋白质组学（metaproteomics）分析的对比结果显示，二者的主要差异在于未知微生物的占比。各反应仓室中检测到的挥发性脂肪酸含量，可反映出微生物对代谢产物的同化利用效能。若能成功整合这两类分析方法，将有助于更深入地理解复杂体系的厌氧消化过程。