Embryologic Origin Influences Smooth Muscle Cell Phenotypic Modulation Signatures in Murine Marfan Syndrome Aortic Aneurysm

Aortic single-cell RNA sequencing data from second heart field lineage traced mice with Fbn1C1041G/+ and control mice. Overall design: Aortic tissue from 16-week-old control and Fbn1C1041G/+ (Marfan syndrome) mice with transgenic second heart field lineage tracing cre-lox model (Nkx2-5-cre) was subjected to tissue digestion and single-cell RNA sequencing. Male and female replicate pools from each genotype were used, samples were processed from lineage positive and negative cell pools, yielding 8 total samples.

携带Fbn1C1041G/+突变的第二心场谱系示踪小鼠与对照小鼠的主动脉单细胞RNA测序（single-cell RNA sequencing）数据。实验设计概述：本研究选取16周龄的对照小鼠及携带Fbn1C1041G/+突变（可诱发马凡综合征）的转基因第二心场谱系示踪cre-lox重组系统（Nkx2-5-cre）模型小鼠的主动脉组织，对其进行组织消化与单细胞RNA测序。每个基因型均设置雌雄两组重复混合样本，分别对谱系阳性与谱系阴性细胞池完成样本制备，最终获得共计8个测序样本。