Targeting PBK/TOPK decreases growth and survival of glioblastoma stem cells in vitro and attenuates tumor growth in vivo

Gene knockdown of PBK led to decreased proliferation and sphere formation in the GSC cultures. Treatment of cells with different concentrations of HI-TOPK-032 almost completely abolished growth and proliferation and elicited a large increase in apoptosis Total RNA isolated from GSC cultures featuring PBK gene knock-down (shRNA) was compared to that from non-silencing control GSC cultures (NS-shRNA). Total RNA isolated from GSC cultures treated with PBK-inhibitor was compared to that from untreated GSC cultures.

PBK基因敲低可导致胶质瘤干细胞（Glioma Stem Cells, GSC）培养物的增殖能力与成球能力下降。采用不同浓度的HI-TOPK-032处理细胞，几乎完全抑制了细胞的生长与增殖，并显著诱导了大量细胞凋亡。从经PBK基因敲低（短发夹RNA, shRNA）处理的胶质瘤干细胞培养物中提取的总RNA，与非沉默对照shRNA（NS-shRNA）处理的胶质瘤干细胞培养物的总RNA进行了比对；从经PBK抑制剂处理的胶质瘤干细胞培养物中提取的总RNA，与未处理的胶质瘤干细胞培养物的总RNA进行了对比。