Primary scaffolds for de novo whole genome sequencing of Syzygium oleosum.

Whole genome sequencing and de novo assembly of the Australian rainforest tree, Syzygium oleosum, using 10x Chromium linked reads. Supernova v2 assembly of the data generated a 431 Mb draft genome (10.35% gaps) with a maximum scaffold size of 5.89 Mb, primary scaffold N50 of 976 kb (L50=98) and a primary scaffold BUSCO (v2.0.1 short) completeness estimate of 93.2% (4.5% missing). Pseudodiploid processing of the assembly generated non-redundant alternative scaffolds for 187 Mb (43.3%) of the assembly, with an N50 of 1.94 Mb (L50=30, 13.35% gaps, 65.1% BUSCO complete; 33.2% BUSCO missing).

利用10x Chromium链接读长测序技术对澳大利亚雨林树种香蒲桃（Syzygium oleosum）开展全基因组测序与从头组装。依托所获数据通过Supernova v2软件完成组装，得到总长431 Mb的草图基因组，其中序列缺口占比10.35%；该组装的最大支架序列长度为5.89 Mb，初级支架N50值为976 kb（L50=98），初级支架的BUSCO（Benchmarking Universal Single-Copy Orthologs）v2.0.1短版本完整度评估结果为93.2%，缺失序列占比4.5%。对该组装结果进行假二倍体处理后，为组装序列中187 Mb（占比43.3%）的区域生成了非冗余替代支架序列，该序列的N50值为1.94 Mb（L50=30），序列缺口占比13.35%，BUSCO完整度为65.1%，缺失序列占比33.2%。