mass spectrometry-based phospho-proteomics data

To identify the regulatory relationship between YBX1 and cellular metabolism, we performed mass spectrometry-based phospho-proteomics assays in two independent YBX1 short hairpin RNA (shRNA)-transduced MDA-MB-231 and in Cre-loxP-induced Ybx1 knockout mouse embryonic fibroblasts (Ybx1-/- MEFs) cells. We quantified 12185 distinct phosphorylation sites on 3547 different proteins

为明确Y盒结合蛋白1（Y-box binding protein 1, YBX1）与细胞代谢之间的调控关系，本研究在两株独立的、转染YBX1短发夹RNA（short hairpin RNA, shRNA）的MDA-MB-231细胞，以及经Cre-loxP系统诱导构建的Ybx1基因敲除小鼠胚胎成纤维细胞（Ybx1-/- MEFs）中开展了基于质谱的磷酸化蛋白质组学检测。本次研究共定量得到3547种不同蛋白质上的12185个独特磷酸化位点。