microRNA expression patterns related to merkel cell polyomavirus infection in human merkel cell carcinoma. Homo sapiens

We performed miRNA expression profiling in a series of human Merkel Cell carcinoma samples using a microarray approach. Significant differentially expressed miRNAs among groups were identified using SAM analysis. Overall design: Agilent microarray platform containing 723 human miRNAs was used to determine miRNA expression profiles in 16 human Merkel cell carcinoma (MCC) samples. To validate the microarray platform, the expression levels of selected miRNAs were evaluated using qRT-PCR.

本研究采用微阵列（microarray）技术，对一系列人默克尔细胞癌样本进行微小RNA（microRNA, miRNA）表达谱分析。借助SAM分析（Significance Analysis of Microarrays），筛选得到各组间存在显著表达差异的微小RNA。实验设计概况：采用搭载723个人类微小RNA探针的安捷伦（Agilent）微阵列平台，对16例人默克尔细胞癌（MCC）样本的微小RNA表达谱进行检测。为验证该微阵列平台的可靠性，采用实时定量逆转录聚合酶链反应（quantitative real-time reverse transcription polymerase chain reaction, qRT-PCR）对筛选出的部分微小RNA的表达水平进行了验证。