Expression data from Arabidopsis sid2.2 mutant (ICS1 mutant) and wild type (Col-0)

The sid2.2 mutant (Dewdney et al. 2000) with deletion/arrangement in exon 9 is deficient for salicylic acid synthesis. We used Affymetrix microarrays to detail the global programme of gene expression underlying metabolic and cellular changes in the rosettes of the Arabidopsis sid2.2 mutant and wild type grown under strong sulfate limitation. Two genotypes: wild type (Col-0) and sid2.2 mutant were grown under low sulfate (S-) 60 days in short day condition. Three consecutive cultures (C1 C2 and C3) were performed at several month interval. For the three cultures and each genotype, rosettes of >6 plants were harvested and mixed to extract RNA.

外显子9存在缺失/重排的sid2.2突变体（Dewdney等，2000年）存在水杨酸合成缺陷。本研究采用Affymetrix基因芯片，详细解析了强硫酸盐胁迫下生长的拟南芥sid2.2突变体与野生型莲座叶中，介导代谢与细胞变化的全局基因表达程序。实验设置两种基因型：野生型（Col-0）与sid2.2突变体，二者均在短日照条件下以低硫酸盐（S-）培养基培养60天。实验分三次连续培养（C1、C2、C3），各培养批次间隔数月。针对三次培养及每种基因型，均收获6株以上植株的莲座叶并混合以提取RNA。