Investigating the links between organic acid and carbohydrate regulation of gene expression.

Arabidopsis acetate non-utilizing mutants were isolated based on fluoroacetate resistant germination. Interestingly, a number of these mutants exhibited altered developmental characteristics in response to exogenous sucrose supply, such as bleaching of the cotyledons. A preliminary microarray experiment has already been conducted on one of the mutants, acn1-2. The gene expression analysis was done using 3 day-old seedlings of acn1-2 and the parent, Col-7, which were germinated on agar plates with and without exogenous sucrose. A cross-comparison of acn1-2 and Col-7 revealed that the expression of a number of carbohydrate responsive genes was altered in the mutant. The request for further microarray analysis is to confirm this result. Keywords: gene_knock_out Number of plants pooled:3 x 1000 seedlings

本研究通过氟乙酸抗性萌发筛选，分离得到拟南芥（Arabidopsis）乙酸非利用突变体。值得注意的是，其中部分突变体在响应外源蔗糖供给时表现出发育异常特征，例如子叶褪色。目前已针对突变体acn1-2完成了一项初步的基因芯片（microarray）实验：该基因表达分析以生长3天的acn1-2及其野生型亲本Col-7幼苗为材料，这些幼苗分别在添加与未添加外源蔗糖的琼脂平板上萌发获得。通过acn1-2与Col-7的交叉比对分析发现，该突变体中多个碳水化合物响应基因的表达发生了改变。本次申请开展进一步的基因芯片分析，旨在验证上述实验结果。关键词：基因敲除（gene knock-out）；混合样本量：3×1000株幼苗