The effects of Arabidopsis genome duplication on the chromatin organization and transcriptional regulation. The effects of Arabidopsis genome duplication on the chromatin organization and transcriptional regulation

we performed Hi-C to probe the nuclear organization patterns after genome duplication in Arabidopsis, In connection with RNA-Seq data and ChIP-Seq data, we found that the transcription of a group of genes is specifically affected by the chromatin interactions with epigenome dynamics. Overall design: Performed Hi-C on autotetraploid and wide type Arabidopsis; examination of 2 different histone modifications H3K4me3 and H3K27me3 in autotetraploid and wilde type Arabidopsis, and RNA-seq also used to check the transcriptional changes in these two samples.

本研究利用高通量染色体构象捕获（Hi-C）技术，探究了拟南芥基因组加倍后的细胞核组织模式。结合RNA测序（RNA-Seq）与染色质免疫共沉淀测序（ChIP-Seq）数据，我们发现一组基因的转录会通过染色质相互作用及表观基因组动态变化受到特异性影响。实验设计：对同源四倍体拟南芥与野生型拟南芥开展Hi-C检测；在两类样本中检测两种组蛋白修饰H3K4me3与H3K27me3，并通过RNA测序分析二者的转录组变化。