Comparison between GT and ntcA-overexpressing strains of cyanobacteria [nitrogen-depleted conditions]. Synechocystis sp. PCC 6803

NtcA regulates primary in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed an ntcA-overexpressing strain, named NOX10, by introducing the ntcA genes fusing psbAII promoter by homologous recombination. The transcript profiles of parental wild-type strain GT and NOX10 were compared by microarray CyanoChip (Takrara bio.). Experiments were performed two times with biologically independent RNA. Overall design: Total RNAs from two independent wild-type (GT) and two independent ntcA-overexpressing strains were differently labeled by Cy3 or Cy5, followed by hybridization on CyanoChip.

NtcA可调控单细胞蓝细菌集胞藻（Synechocystis sp. PCC6803）的初级代谢过程。本研究通过同源重组方法引入融合psbAII启动子的ntcA基因，构建得到ntcA过表达菌株NOX10。采用宝生物工程（Takara Bio）的CyanoChip微阵列芯片对亲本野生型菌株GT与NOX10的转录谱进行比较分析。本实验共开展两次，每次均使用独立的生物学重复RNA样本。实验设计概述：将两份独立野生型（GT）菌株与两份独立ntcA过表达菌株的总RNA分别用Cy3或Cy5进行荧光标记，随后将标记产物在CyanoChip芯片上进行杂交。