Dataset for "Precise 3D Tracking of Highly Non-planar Eukaryotic Flagellar Beating Patterns using Digital Holographic Microscopy"

This repository contains all data supporting the publication "Precise 3D Tracking of Highly Non-planar Eukaryotic Flagellar Beating Patterns using Digital Holographic Microscopy" The dataset comprises three core components: Tracking Results High-resolution tracking outputs for two cells, Reclinomonas americana and mouse spermatozoa, captured using the holoV3C algorithm. Each dataset is saved in an Excel file where individual worksheets correspond to sequential video frames, containing (x,y,z) coordinates of tracked flagellar points. Axial Resolution Assessment Derived 3D tracking data for two test objects: 1 μm polystyrene research particles and mouse spermatozoa flagella. Frame-by-frame coordinate sequences alongside consolidated metrics quantifying axial resolution performance across both samples. Processing Time Logs Detailed timing records for each stage of the 3D flagellum tracking pipeline applied to mouse spermatozoa data. Enables assessment of computational performance and identification of processing bottlenecks. For access to raw data and holograms, please contact us. Description of each data file: data S1.Results of 3D tracking of mouse spermatozoa flagellum. Processed (x,y,z) coordinates for each frame of the analyzed sequence are provided in individual worksheets, named by frame number. The “metadata” worksheet details the experimental settings. The “all_data” worksheet concatenates the full time series of 3D coordinates for the analyzed sequence.data S2.Results of 3D tracking of R. americana flagellum. Processed (x,y,z) coordinates for each frame of the analyzed sequence are provided in individual worksheets, named by frame number. The “metadata” worksheet details the experimental settings. The “all_data“ worksheet concatenates the full time series of 3D coordinates for the analyzed sequence.data S3.Processing time logs for lateral and axial localization in the mouse spermatozoa flagellum 3D tracking pipeline. For each frame, the elapsed times (s) of each core stage of the tracking pipeline are recorded. The worksheet summarizes average and standard deviation for each stage across the full sequence. The “metadata” worksheet details the experimental settings.data S4.Three-dimensional coordinates of the mouse spermatozoa flagellum used for the axial resolution analysis. Processed (x,y,z) coordinates for each frame of the analyzed sequence are provided in individual worksheets, named by frame number. The “metadata” worksheet details the experimental settings.data S5.Three-dimensional coordinates of the dispersed polystyrene 1.05-μm research particles used for the axial resolution analysis. Processed (x,y,z) coordinates for each frame of the analyzed sequence are provided in individual worksheets, named by frame number. The “metadata” worksheet details the experimental settings.data S6.Axial resolution metrics. Consolidated performance table summarizing root-mean-square error (RMSE), mean bias error (MBE), and maximum error in axial localization for both test objects - mouse spermatozoa flagellum (data S4) and polystyrene particles (data S5). All metrics are reported in micrometers. The “metadata” worksheet details the experimental settings.本仓库包含支撑论文《利用数字全息显微镜（Digital Holographic Microscopy）精准追踪高度非平面真核鞭毛运动模式》的全部数据集。 本数据集包含三大核心组成部分： #### 追踪结果 针对美洲肾鞭毛虫（Reclinomonas americana）与小鼠精子两种样本，采用holoV3C算法获取的高分辨率追踪输出结果。每份数据集均存储于Excel文件中，文件内的独立工作表对应连续的视频帧，其中包含已追踪鞭毛点的(x,y,z)三维坐标。 #### 轴向分辨率评估 针对两类测试对象衍生的三维追踪数据：1 μm聚苯乙烯研究颗粒与小鼠精子鞭毛。包含逐帧坐标序列，以及用于量化两类样本轴向分辨率性能的综合指标。 #### 处理时长日志 针对小鼠精子鞭毛三维追踪流程各阶段的详细计时记录，可用于评估计算性能并定位处理瓶颈。 如需获取原始数据与全息图，请联系我们。 ### 各数据文件详细说明 1. data S1：小鼠精子鞭毛三维追踪结果。分析序列各帧的处理后(x,y,z)坐标存储于以帧号命名的独立工作表中。"metadata（元数据）"工作表记录了实验设置信息；"all_data"工作表整合了本次分析序列的完整三维坐标时间序列。 2. data S2：美洲肾鞭毛虫（Reclinomonas americana）鞭毛三维追踪结果。分析序列各帧的处理后(x,y,z)坐标存储于以帧号命名的独立工作表中。"metadata（元数据）"工作表记录了实验设置信息；"all_data"工作表整合了本次分析序列的完整三维坐标时间序列。 3. data S3：小鼠精子鞭毛三维追踪流程中横向与轴向定位的处理时长日志。记录了每帧追踪流程各核心阶段的耗时（单位：秒），对应工作表汇总了全序列各阶段的平均值与标准差。"metadata（元数据）"工作表记录了实验设置信息。 4. data S4：用于轴向分辨率分析的小鼠精子鞭毛三维坐标。分析序列各帧的处理后(x,y,z)坐标存储于以帧号命名的独立工作表中。"metadata（元数据）"工作表记录了实验设置信息。 5. data S5：用于轴向分辨率分析的分散态1.05 μm聚苯乙烯研究颗粒的三维坐标。分析序列各帧的处理后(x,y,z)坐标存储于以帧号命名的独立工作表中。"metadata（元数据）"工作表记录了实验设置信息。 6. data S6：轴向分辨率指标。综合性能表汇总了两类测试对象——小鼠精子鞭毛（对应data S4）与聚苯乙烯颗粒（对应data S5）的轴向定位均方根误差（root-mean-square error, RMSE）、平均偏差误差（mean bias error, MBE）与最大误差，所有指标单位均为微米。"metadata（元数据）"工作表记录了实验设置信息。