Quant-seq analysis of differential gene expression and polyadenylation site usages in caused by inhibition of CDK12 and CDK13 using THZ531 in THP-1 cells (3' Quant-seq)

We used 3' RNA-Seq to determine effect of dual inhibition of CDK12 and CDK13 on gene expression and polyadenatltion site usages in THP-1 cells Overall design: we performed 3'RNA-seq (Quantseq) on 6 hours THZ531 or DMSO reatment on parental THP-1 cells

本研究采用3'端RNA测序（3' RNA-Seq）技术，旨在明确CDK12与CDK13双重抑制对THP-1细胞内基因表达及多聚腺苷酸化位点使用情况的影响。实验设计：对亲本THP-1细胞施以6小时的THZ531或二甲基亚砜（DMSO）处理后，通过QuantSeq平台开展3'端RNA测序。