lncRNA expression analysis in patients with eosinophilic and neutrophilic asthma

Long non-coding RNA (lncRNA) are known to be important in many diseases. There has been some reports that lncRNA take part in the pathogenesis of systemic inflammation of asthma. lncRNA regulates gene transcription, protein expression and epigenetic regulation. However, the lncRNAs associated with differently airway phenotype such as eosinophilic and neutrophilic asthma remains unknown. We aimed at identifying the differences in circulating lncRNA signature in Eos and Neu samples. lncRNA expression were studied between blood samples from Eos patients, Neu patients and healthy individuals (Control). Bioinformatic analysis was used to describe relevant biological pathways. Using quantitative real-time PCR, lncRNA expression was measured. Comparing with Control samples, Eos sample has 190 own lncRNA and Neu sample has 166 own lncRNA(difference = 2-fold). KEGG pathway annotation data and GO terms revealed that several lncRNAs are possibly associated with respective phenotype. lncRNA was identifying to differ significantly between Eos and Neu samples by using qRT-PCR. The results show us that lncRNA may be involved in different phenotypes of asthma. Whether we can recognize different phenotypes of asthma through these lncRNA (as biomarkers) needs further study. Overall design: Patients with eosinophilic asthma (Eos, n = 12) and neutrophilic asthma (Neu, n = 6) were selected in the study according the accepted standard (Eos: induced sputum eosinophil count >3% and neutrophils <63%; Neu: induced sputum eosinophil count <3% and neutrophils>63% )1. Exclusion criteria contained recent (within the past weeks) respiratory tract infection, recent unstable asthma, recent asthma exacerbation, current smoking (or a history of smoking, within 6 months of cessation), and changes in maintenance therapy. All participants were selected from the People's Liberation Army General Hospital, and all participants have been given informed consent before their inclusion. Healthy individuals were selected as Control samples (n = 6). The results show us that lncRNA may be involved in different phenotypes of asthma. Whether we can recognize different phenotypes of asthma through these lncRNA (as biomarkers) needs further study. This dataset is related to GSE106230

长链非编码RNA（long non-coding RNA，lncRNA）在多种疾病中发挥重要作用。已有多项研究报道，lncRNA参与哮喘全身性炎症的发病机制。lncRNA可调控基因转录、蛋白质表达及表观遗传调控。然而，与嗜酸性粒细胞性哮喘、中性粒细胞性哮喘等不同气道表型相关的lncRNA仍尚未明确。本研究旨在鉴定嗜酸性粒细胞性哮喘（Eos）与中性粒细胞性哮喘（Neu）样本中循环lncRNA特征谱的差异。

本研究对比分析了嗜酸性粒细胞性哮喘患者、中性粒细胞性哮喘患者及健康个体（对照组）的血液样本中的lncRNA表达情况。采用生物信息学方法解析相关生物学通路，并通过定量实时聚合酶链反应（quantitative real-time PCR，qRT-PCR）检测lncRNA的表达水平。

与对照组样本相比，嗜酸性粒细胞性哮喘样本存在190种特异性lncRNA，中性粒细胞性哮喘样本存在166种特异性lncRNA（表达差异倍数达2倍）。京都基因与基因组百科全书通路（Kyoto Encyclopedia of Genes and Genomes，KEGG）注释数据及基因本体论（Gene Ontology，GO）术语分析显示，多种lncRNA可能与对应哮喘表型相关。经qRT-PCR验证发现，嗜酸性粒细胞性哮喘与中性粒细胞性哮喘样本间的lncRNA表达存在显著差异。

研究结果表明，lncRNA可能参与哮喘的不同表型进程。能否通过这些lncRNA作为生物标志物识别哮喘的不同表型，仍需进一步研究。

总体实验设计：本研究根据公认标准纳入嗜酸性粒细胞性哮喘患者（Eos组，n=12）及中性粒细胞性哮喘患者（Neu组，n=6）：嗜酸性粒细胞性哮喘患者的诱导痰嗜酸性粒细胞计数＞3%且中性粒细胞计数＜63%；中性粒细胞性哮喘患者的诱导痰嗜酸性粒细胞计数＜3%且中性粒细胞计数＞63%¹。排除标准包括：近期（数周内）呼吸道感染、近期哮喘病情不稳定、近期哮喘急性加重、当前吸烟（或6个月内有吸烟史且已戒烟）以及维持治疗方案发生变更。所有研究对象均选自中国人民解放军总医院，且入组前均已签署知情同意书。健康个体作为对照组（n=6）。

研究结果再次表明，lncRNA可能参与哮喘的不同表型进程。能否通过这些lncRNA作为生物标志物识别哮喘的不同表型，仍需进一步研究。

本数据集与GSE106230相关。