CD177+ neutrophils restrict neuroinflammation following CNS injury

Neutrophils are the most abundant circulating leukocyte population that play critical roles in neuroinflammation after ischemic stroke. CD177, a glycoprotein on neutrophils, is emerging as an important immune regulator which fundamentally affects multiple inflammatory diseases. However, the role and regulatory mechanism of CD177 protein of neutrophils in neuroinflammation remains elusive. Using single-cell RNA sequencing (scRNA-Seq), we characterized CD177high neutrophils as a novel anti-inflammatory subset in response to ischemic stroke. Stroke model was induced by middle cerebral artery occlusion (MCAO). scRNA-seq was performed on bone marrow cells from mice after stroke and control conditions.

中性粒细胞（Neutrophils）是体内丰度最高的循环白细胞群，在缺血性脑卒中（ischemic stroke）后的神经炎症（neuroinflammation）过程中发挥关键作用。CD177是表达于中性粒细胞表面的糖蛋白（glycoprotein），作为新兴的重要免疫调节因子，可对多种炎症性疾病产生根本性调控效应。然而，中性粒细胞表面的CD177蛋白在神经炎症中的作用及调控机制仍尚不明确。本研究通过单细胞RNA测序（single-cell RNA sequencing，scRNA-Seq）技术，将CD177高表达中性粒细胞鉴定为一类响应缺血性脑卒中的新型抗炎细胞亚群。研究采用大脑中动脉闭塞（middle cerebral artery occlusion, MCAO）法构建脑卒中模型，并对模型组与对照组小鼠的骨髓细胞开展scRNA-seq测序分析。