PB-Cre/Pten/Smad4 mouse prostate cancer micorarray for CRPC and lung metastasis studies. PB-Cre/Pten/Smad4 mouse prostate cancer micorarray for CRPC and lung metastasis studies

PB-Cre/Pten/Smad4 is a transgenic mouse model of metastatic prostate adenocarcinoma (PMID: 21289624). To study the transcriptomic alterations associated with castration-resistant prostate cancer (CRPC), the PB-Cre/Pten/Smad4 males with established prostate cancer were treated with surgical castration followed by enzalutamide-admixed diet. After about 4 weeks, dorsolateral prostate (DLP) lobes of treatment-naïve prostate tumors (N=2) and CRPC tumors (N=3) were harvested and extracted for RNA purification and microarray profiling. To further study the transcriptomic changes associated with lung metastases of the PB-Cre/Pten/Smad4/mTmG CRPC model, the PB-Cre/Pten/Smad4 males with established prostate cancer were treated with surgical castration followed by enzalutamide-admixed diet. About 3 months later, from one mouse anterior prostate (AP), dorsolateral prostate (DLP), ventral prostate (VP) and GFP+ lung metastasis nodules were each harvested for RNA purification and microarray profiling. Overall design: The samples were all harvested from PB-Cre/Pten/Smad4 male mice. For the study of CRPC, treatment-naïve prostate tumors (N=2) and CRPC tumors (N=3) were profiled. For the study of CRPC primary tumor and lung metastasis, 1 AP sample, 1 DLP sample, 1 VP sample and 1 lung metastasis sample were profiled, all from one mouse.

PB-Cre/Pten/Smad4是一种转移性前列腺腺癌转基因小鼠模型（PubMed编号：21289624）。为探究与去势抵抗性前列腺癌（castration-resistant prostate cancer, CRPC）相关的转录组改变，研究者对成瘤的PB-Cre/Pten/Smad4雄性小鼠行手术去势，随后喂食掺有恩扎卢胺（enzalutamide）的饲料。约4周后，采集未接受治疗的前列腺肿瘤样本（N=2）与CRPC肿瘤样本（N=3）的背外侧前列腺（dorsolateral prostate, DLP）组织，提取RNA并开展微阵列基因表达谱分析。为进一步研究PB-Cre/Pten/Smad4/mTmG CRPC模型的肺转移相关转录组变化，研究者再次对成瘤的PB-Cre/Pten/Smad4雄性小鼠行手术去势，随后喂食掺有恩扎卢胺的饲料。约3个月后，从1只小鼠的前前列腺（anterior prostate, AP）、背外侧前列腺（DLP）、腹侧前列腺（ventral prostate, VP）以及GFP阳性肺转移结节中分别获取样本，提取RNA并开展微阵列基因表达谱分析。总体设计：所有样本均来源于PB-Cre/Pten/Smad4雄性小鼠。针对CRPC的研究中，共对未接受治疗的前列腺肿瘤样本（N=2）与CRPC肿瘤样本（N=3）进行了基因表达谱芯片分析。针对CRPC原发肿瘤与肺转移的研究中，从同1只小鼠中分别采集1份前前列腺样本、1份背外侧前列腺样本、1份腹侧前列腺样本及1份肺转移样本，均进行了基因表达谱芯片分析。