ERG and c-MYC Co-regulate a Critical Gene Network in BCR-ABL1 B-cell Acute Lymphoblastic Leukemia. ERG and c-MYC Co-regulate a Critical Gene Network in BCR-ABL1 B-cell Acute Lymphoblastic Leukemia

Philadelphia chromosome-positive B-cell acute lymphoblastic leukaemia (Ph+B-ALL), characterised by the BCR-ABL1 fusion gene, remains a poor prognosis cancer that needs new therapeutic approaches. Transcriptomic profiling and pathway analysis identified up-regulation of the oncogenic transcription factors ERG and c-MYC, with enrichment of genes involved in metabolic processes including ribosome biogenesis. Overall design: Comparative gene expression profiling analysis of RNA-seq data from defined cell populations. ChIP-seq from C57BL/6 proB cells and Rag1CreT/+;ErgΔ/Δ preproB cells.

以BCR-ABL1融合基因为特征的费城染色体阳性B细胞急性淋巴细胞白血病（Philadelphia chromosome-positive B-cell acute lymphoblastic leukaemia, Ph+B-ALL）仍是一类预后不良的恶性肿瘤，亟需开发新型治疗策略。转录组表达谱分析（transcriptomic profiling）与通路分析结果显示，致癌转录因子ERG与c-MYC的表达显著上调，且涉及核糖体生物发生等代谢过程的基因显著富集。整体实验设计：对明确分类的细胞群体的RNA测序（RNA-seq）数据开展对比基因表达谱分析；同时对C57BL/6原B细胞（proB cells）及Rag1CreT/+;ErgΔ/Δ前原B细胞（preproB cells）进行染色质免疫沉淀测序（ChIP-seq）分析。