RNA sequencing of HCT116 and HKE3 colorectal cancer cell lines before and after stimulation with TGF-alpha. RNA sequencing of HCT116 and HKE3 colorectal cancer cell lines before and after stimulation with TGF-alpha

RNA sequencing was performed on HCT116 and HKE3 colorectal cancer cell lines before stimulation and at 15, 30, 60, 90 and 120 mins post-stimulation with transforming growth factor alpha (TGF-α) (0.01 µg/mL, Abcam). Three replicates were sequenced at each time point. Overall design: RNAseq profiles from HCT116 and HKE3 cells before and after stimulation with TGF-alpha. In total, 36 cDNA libraries (2 cell lines X 6 time-points X 3 replicates per timepoint) were generated for sequencing.

本研究对HCT116与HKE3结直肠癌细胞系，在转化生长因子α（transforming growth factor alpha, TGF-α，0.01 µg/mL，购自Abcam）刺激前，以及刺激后15、30、60、90、120分钟的样本开展RNA测序。每个时间点设置3次生物学重复用于测序。整体实验设计：获取经TGF-α刺激前后的HCT116与HKE3细胞的RNA测序表达谱。本次实验共构建36个cDNA文库（2种细胞系 × 6个时间点 × 每个时间点3次重复）用于测序。