Molecular characterization of antimicrobial resistant Pseudomonas aeruginosa isolates in tertiary care hospital, Kathmandu

Molecular characterizing Pseudomonas aeruginosa isolates that are resistant to antibiotics in a tertiary care hospital, like one in Kathmandu, means figuring out the exact genetic factors and mechanisms that cause the resistance. The study collects samples, tests them for antimicrobial susceptibility (AST), and then uses Clinical and Laboratory Standards Institute (CLSI) guidelines to decide whether the isolates are susceptible, intermediate, or resistant.Molecular characterization involves detecting resistance genes using PCR, gene sequencing, and plasmid analysis. Genotyping involves pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and whole genome sequencing (WGS). Data analysis includes assessing the prevalence of resistance to different antibiotics, examining the association with clinical outcomes, and identifying epidemiological trends in the spread of resistant strains within the hospital.Conclusions and recommendations include infection control measures, antibiotic stewardship, and further research. The findings could help in understanding the molecular mechanisms behind antimicrobial resistance in Pseudomonas aeruginosa in a specific hospital setting, aiding in the development of targeted interventions to control the spread of resistant strains. Further research could investigate resistance mechanisms in greater detail or alternative treatment options.

针对三级医院（如加德满都的某家医院）内的抗生素耐药铜绿假单胞菌（Pseudomonas aeruginosa）分离株开展分子特征分析，其核心为明确导致该类菌株耐药性的确切遗传因素与作用机制。本研究通过采集样本、开展抗菌药物敏感性试验（AST），并依据美国临床和实验室标准协会（Clinical and Laboratory Standards Institute, CLSI）指南，判定分离株的药敏结果为敏感、中介或耐药。分子特征分析环节包括采用聚合酶链式反应（PCR）、基因测序及质粒分析检测耐药基因；基因分型则涵盖脉冲场凝胶电泳（PFGE）、多位点序列分型（MLST）与全基因组测序（WGS）。数据分析环节包括评估不同抗菌药物的耐药率、分析耐药性与临床结局的关联，以及明确医院内耐药菌株传播的流行病学趋势。研究结论与建议涵盖感染防控措施、抗菌药物管理及后续研究方向，本研究结果有助于阐明特定医院环境下铜绿假单胞菌抗菌耐药性的分子机制，为制定管控耐药菌株传播的针对性干预方案提供支撑；未来可进一步深入探究耐药机制或开发替代治疗方案。