Gene expression profile at single-cell level of hematopoietic stem and progenitor cells (HSPCs) from individuals with chronic myelomonocytic leukemia (CMML)

Myeloblast expansion is a hallmark of disease progression in leukemias and comprises of CD34+ hematopoeitic stem and progeneitor cells. We used single-cell RNA sequencing (scRNAseq) to analyze the transcriptional states of HSPCs in CMML. CD34+ HSPCs were isolated from bone marrow mononuclear cells from patients diagnosed with CMML and transcriptionally profiled using scRNAseq.

成髓细胞扩增是白血病疾病进展的标志性特征，其细胞群体由CD34+造血干祖细胞（hematopoietic stem and progenitor cells）构成。本研究借助单细胞RNA测序（single-cell RNA sequencing，scRNAseq）技术，对慢性粒单核细胞白血病（Chronic Myelomonocytic Leukemia，CMML）患者体内的造血干祖细胞（hematopoietic stem and progenitor cells，HSPCs）转录状态进行了分析。我们从确诊为CMML的患者骨髓单个核细胞中分离得到CD34+ HSPCs，并通过scRNAseq完成了其转录谱分析。