Di-GRAPH sgs1∆ dataset

The Di-GRAPH sgs1∆ dataset contains paired-end FASTQ files obtained from S.cerevisiae PMV sgs1∆ cells at 4 different timepoints: T0 (Timepoint 0, data prior to DSB induction), TSG (Timepoint Short Galactose, data from non-selected survivors), TLG (Timepoint Long Galactose, data from selected survivors) and TLR (Timepoint Long Raffinose, data from undamaged cells); and 3 replicates defined as E1 (Experiment 1), E2 (Experiment 2) and E3 (Experiment 3).

This dataset can be used for test run of the Di-GRAPH bioinformatic workflow available at https://github.com/acb-lab/Di_GRAPH

Di-GRAPH sgs1Δ数据集包含双端FASTQ格式测序文件，其测序数据来源于酿酒酵母（Saccharomyces cerevisiae）PMV菌株的sgs1Δ缺失突变体细胞，涵盖4个不同时间点的样本：T0（时间点0，即双链断裂（Double-Strand Break, DSB）诱导前的样本数据）、TSG（半乳糖短时诱导时间点，对应未筛选存活体的样本数据）、TLG（半乳糖长时诱导时间点，对应筛选后存活体的样本数据）以及TLR（棉籽糖长时培养时间点，对应未受损伤细胞的样本数据）；该数据集同时包含3次生物学重复，分别标记为E1（实验1）、E2（实验2）与E3（实验3）。该数据集可用于对部署于https://github.com/acb-lab/Di_GRAPH 的Di-GRAPH生物信息学分析流程开展测试运行。