RNA Seq analysis of WT vs rv3249c mutant

We report the characterization of an Mtb transcriptional regulator Rv3249c (AlkX). Combined, our results define the primary role of AlkX as a transcriptional repressor of the alkBrubAB operon and suggests the operon contributes to intracellular survival of the pathogen and potential persistence phenotypes. RNAseq analysis of was performed on the wild-type H37Rv and mutant strains of M. tuberculosis grown planktonically

本研究报道了结核分枝杆菌（Mtb）转录调控因子Rv3249c（AlkX）的功能表征。综合全部实验结果，本研究明确了AlkX作为alkBrubAB操纵子（alkBrubAB operon）的转录阻遏蛋白的核心功能，并提示该操纵子参与病原菌的胞内存活过程及潜在持留表型调控。本研究针对以浮游状态培养的野生型H37Rv与结核分枝杆菌突变菌株开展了转录组测序（RNAseq）分析。