Insulin resistance in 3T3-L1 adipose cells. Mus musculus

3T3-L1 adipose cells were grown, differentiated and insulin resistance was stimulated by addition of TNF or treatment inlcuding chronic insulin and dexamethasone. Overall design: Comparison of gene expression in 3T3-L1 cells during treatment with TNF, chronic insuline and dexamethosone; 2-3 samples per group serve as experimental replicates.

本研究对3T3-L1脂肪细胞（3T3-L1 adipose cells）进行培养与诱导分化，并通过添加肿瘤坏死因子（TNF），或采用联合慢性胰岛素与地塞米松（Dexamethasone）的处理方式，诱导细胞产生胰岛素抵抗。
整体实验设计：对比3T3-L1细胞经TNF、慢性胰岛素联合地塞米松处理后的基因表达差异；每组设置2~3份样本作为实验重复样本。