08.06.2022_SourceFile_RNA-seq.xlsx

In heterogeneous head and neck cancer (HNC), subtype-specific treatment regimens are currently missing. An integrated analysis of patient HNC subtypes using single-cell sequencing and proteome profiles revealed a hallmark epithelial-mesenchymal transition (EMT) signature within the epithelial cancer-cell population. The EMT signature was evident in the invasive mesenchymal subtype and importantly, coincided with PI3K/mTOR pathway inactivation. Conversely, the signature was suppressed in epithelial cancer cells of the basal subtype which exhibits hyperactive PI3K/mTOR oncogenic signalling.

在异质性头颈癌（head and neck cancer, HNC）中，目前尚未建立亚型特异性治疗方案。研究人员通过整合分析患者头颈癌亚型的单细胞测序与蛋白质组图谱数据，在上皮癌细胞群体中鉴定出标志性的上皮间质转化（epithelial-mesenchymal transition, EMT）特征。该EMT特征在侵袭性间质亚型中显著表达，且尤为重要的是，其与PI3K/mTOR信号通路的失活状态相吻合。与之相反，该EMT特征在基底亚型的上皮癌细胞中受到抑制，而该基底亚型恰好呈现高活性的PI3K/mTOR致癌信号通路。