Comparative analysis of cartilage tissue from ANP32A knockout mice and wildtype C57/Bl6 mice. Comparative analysis of cartilage tissue from ANP32A knockout mice and wildtype C57/Bl6 mice

A genetic association between the ANP32A gene and osteoarthritis has been suggested. We compared transcriptome profiles of the articular cartilage and subchondral bone from mice deficient in ANP32A with wild-type mice to get insights into the role of ANP32A in the pathogenesis of ostearthritis. Overall design: The articular cartilage and subchondral bone from the tibial plateau of the mouse knee joint was carefully dissected in one piece from 8-week-old ANP32 knockout mice and wild-type controls

已有研究提示ANP32A基因（ANP32A gene）与骨关节炎（osteoarthritis）存在遗传关联。本研究对比了ANP32A敲除小鼠与野生型（wild-type）小鼠的关节软骨（articular cartilage）及软骨下骨（subchondral bone）的转录组谱（transcriptome profiles），以阐明ANP32A在骨关节炎发病机制中的作用。实验整体设计如下：从8周龄的ANP32A敲除小鼠及野生型对照小鼠的膝关节胫骨平台处，完整剥离得到一体式的关节软骨与软骨下骨组织。