Data_Sheet_3_Deep Sequencing of Plasma Exosomal microRNA Level in Psoriasis Vulgaris Patients.XLS

Psoriasis is a chronic skin disease affecting 1% to 3% of the world population. Psoriasis vulgaris (PV) is the most common form of psoriasis. PV patients suffer from inflamed, pruritic and painful lesions for years (even a lifetime). However, conventional drugs for PV are costly. Considering the need for long-term treatment of PV, it is urgent to discover novel biomarkers and therapeutic targets. Plasma exosomal miRNAs have been identified as the reliable biomarkers and therapy targets of human diseases. Here, we described the levels of plasma exosomal miRNAs in PV patients and analyzed the functional features of differently expressed miRNAs and their potential target genes for the first time. We identified 1,182 miRNAs including 336 novel miRNAs and 246 differently expressed miRNAs in plasma exosomes of healthy people and PV patients. Furthermore, the functional analysis found differently expressed miRNA-regulated target genes enriched for specific GO terms including primary metabolic process, cellular metabolic process, metabolic process, organic substance metabolic process, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway containing cellular processes, human diseases, metabolic pathways, metabolism and organismal systems. In addition, we found that some predicted target genes of differentially expressed miRNAs, such as CREB1, RUNX2, EGFR, are both involved in inflammatory response and metabolism. In summary, our study identifies many candidate miRNAs involved in PV, which could provide potential biomarkers for diagnosis of PV and targets for clinical therapies against PV.

银屑病（Psoriasis）是一种慢性皮肤病，全球约1%至3%的人口受其困扰。寻常型银屑病（Psoriasis vulgaris, PV）是银屑病最为常见的临床类型，PV患者会长期（甚至终身）遭受炎症性、瘙痒且伴疼痛的皮损折磨。然而，当前用于PV治疗的常规药物成本高昂，鉴于PV需长期治疗的临床需求，亟需发掘新型生物标志物与治疗靶点。血浆外泌体微RNA（miRNAs）已被证实可作为人类疾病可靠的生物标志物与治疗靶点。本研究首次报道了PV患者血浆外泌体miRNAs的表达水平，并对差异表达miRNAs的功能特征及其潜在靶基因展开分析。本研究在健康人群与PV患者的血浆外泌体中共鉴定出1182种miRNAs，其中包含336种新型miRNAs与246种差异表达miRNAs。进一步功能分析显示，差异表达miRNA调控的靶基因显著富集于特定基因本体（Gene Ontology, GO）术语，涵盖初级代谢过程、细胞代谢过程、代谢过程、有机物质代谢过程；同时富集于京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路，包括细胞过程、人类疾病、代谢通路、新陈代谢及机体系统。此外，本研究发现部分差异表达miRNA的预测靶基因（如CREB1、RUNX2、EGFR）同时参与炎症反应与代谢过程。综上，本研究鉴定出多种参与PV发病过程的候选miRNAs，可为PV的临床诊断提供潜在生物标志物，并为PV的临床治疗提供全新靶点。