A chromosome-scale assembly of the smallest Dothideomycete genome reveals a unique genome compaction mechanism in filamentous fungi. A chromosome-scale assembly of the smallest Dothideomycete genome reveals a unique genome compaction mechanism in filamentous fungi

RNA sequencing was applied to compare the transcriptome profiles of mycelium on potato dextrose (PD) for 5days, mycelium on PD for 15days and mycelium on PD + PEG6000 for 15days, Overall design: For each sample type, independent librarie was generated, sequencing was performed on an Illumina NextSeq 500 platform

本研究采用RNA测序（RNA sequencing）技术，对比三类样本的转录组表达谱：分别为在马铃薯葡萄糖（PD）培养基上培养5天的菌丝体、PD培养基上培养15天的菌丝体，以及添加聚乙二醇6000（PEG6000）的PD培养基上培养15天的菌丝体。整体实验设计：针对每一类样本，均构建独立测序文库，并通过Illumina NextSeq 500测序平台完成测序。