Pond water SIP fraction microbial communities from Walnut Creek, CA, United States - JGIPond_D_40pct_30ng_2_1.7195 metagenome. pond metagenome

SIP metagenome fraction of pond sample from Joint Genome Institute, Walnut Creek spiked with Pseudomonas putida & Escherichia coli bacterial isolates (for each isolate 30ng of DNA labeled at 40pct with C13 were added); replicate number 2. This fraction has a SIP fraction number 11 and DNA density of 1.7195 g/uL. This is part of a larger study to identify the limit of detection of JGI SIP metagenomics pipeline.

本样本为联合基因组研究所（Joint Genome Institute）核桃溪池塘样本的稳定同位素探针（Stable Isotope Probing, SIP）宏基因组组分，掺入了恶臭假单胞菌（Pseudomonas putida）与大肠杆菌（Escherichia coli）细菌分离株：针对每株分离株，均添加30纳克经C13标记40%的DNA；该样本为重复编号2，其SIP组分编号为11，DNA密度为1.7195 g/μL。本样本属于一项旨在评估JGI稳定同位素探针宏基因组分析流程检出限的大型研究的组成部分。