A long-term feeder-free culture method for human pancreatic progenitors on extra cellular matrix or matrix-free polymers. A long-term feeder-free culture method for human pancreatic progenitors on extra cellular matrix or matrix-free polymers

The dataset comprises RNA-seq information of human embryonic stem cell derived pancreatic progenitors and their proliferating cells cultured in defined condition. Total RNA of each sample was isolated, labelled, and purified by standard chemistry. Sequencing libraries were prepared from 250ng of purified total mRNA using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). Single-read RNA-sequencing was performed using the NextSeq 500 (Illumina) with 75 High Output Kit (Illumina, FC-404-2005).

本数据集包含源自人类胚胎干细胞的胰腺祖细胞及其在限定培养条件下增殖的细胞的RNA测序（RNA-seq）信息。所有样本的总RNA均通过标准化学方法完成分离、标记与纯化。使用NEBNext Ultra II RNA文库制备试剂盒（适配Illumina测序平台，NEB），从250ng纯化后的总mRNA中构建测序文库。采用搭载75通量高产出试剂盒（Illumina，货号FC-404-2005）的NextSeq 500测序平台（Illumina）完成单端RNA测序。