Causal Association Between PDCD5 Expression and the Risk of Biliary System Cancers: A Two-Sample Mendelian Randomization Study Based on Peripheral Blood and Immune Single-Cell eQTLs With Multi-Cohort Validation

Supplementary Information Supplementary File 1: Summary of the paraptosis candidate gene set and exposure-side eQTL/single-cell eQTL information Supplementary File 2: Complete results and robustness analyses from the initial MR screen in the eQTLGen blood cis-eQTL stage Supplementary File 3: Construction of instrumental variables and LD clumping details for GTEx v10 whole-blood eQTLs Supplementary File 4: Complete MR outputs and sensitivity analyses from the GTEx v10 whole-blood eQTL replication stage Supplementary File 5: Multi-cohort GEO differential expression analyses (genome-wide DEG results and PDCD5 validation information) Supplementary File 6: Construction, LD processing, and harmonization of PDCD5 immune single-cell sc(e)QTL instruments (for single-cell MR analyses) Supplementary File 7: Cell type-specific OneK1K immune single-cell sc(e)QTL-MR results and sensitivity analyses补充材料 补充文件1：副凋亡（paraptosis）候选基因集汇总及暴露侧表达数量性状位点（expression quantitative trait locus，eQTL）/单细胞表达数量性状位点（single-cell expression quantitative trait locus，sc-eQTL）信息 补充文件2：eQTLGen血液顺式表达数量性状位点（cis-expression quantitative trait locus，cis-eQTL）阶段初始孟德尔随机化（Mendelian Randomization，MR）筛选的完整结果及稳健性分析 补充文件3：基因型组织表达（Genotype-Tissue Expression，GTEx）v10版本全血eQTL的工具变量构建及连锁不平衡（linkage disequilibrium，LD）聚类细节 补充文件4：GTEx v10版本全血eQTL验证阶段的孟德尔随机化（MR）完整输出结果及敏感性分析 补充文件5：多队列基因表达综合（Gene Expression Omnibus，GEO）差异表达分析（全基因组差异表达基因（differentially expressed gene，DEG）结果及程序性细胞死亡因子5（programmed cell death 5，PDCD5）验证信息） 补充文件6：PDCD5免疫细胞单细胞sc(e)QTL工具变量的构建、连锁不平衡（LD）处理及标准化整合（用于单细胞孟德尔随机化分析） 补充文件7：细胞类型特异性OneK1K免疫细胞单细胞sc(e)QTL-MR结果及敏感性分析