Single-cell transcriptome profiles of wild-type and IRF8KO microglia [scRNA-seq]

The role of the lineage-determining transcription factor IRF8 in microglia remains unelucidated. We analyzed the transcriptome diversity of wild-type and IRF8KO microglia. Deep-sequencing and subsequent differential analysis revealed the significance of the IRF8-dependent transcription on microglial heterogeneity and self-renewing. This study provides a novel insight into understanding IRF8-mediated transcriptional programs in microglia. Overall design: Transcriptome profiles of adult WT and IRF8KO microglia were analyzed at a single-cell level using 10xGenomics.

谱系决定转录因子（lineage-determining transcription factor）干扰素调节因子8 (IRF8) 在小胶质细胞（microglia）中的作用仍未阐明。我们对野生型与IRF8敲除小胶质细胞的转录组多样性展开了分析。通过深度测序与后续差异分析，揭示了IRF8依赖型转录对小胶质细胞异质性及自我更新的关键意义。本研究为理解小胶质细胞中IRF8介导的转录程序提供了全新视角。实验整体设计：采用10xGenomics技术在单细胞水平分析成年野生型与IRF8敲除小胶质细胞的转录组谱。