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Genome-wide chromatin and gene expression profiling during memory formation and maintenance in adult mice. [Data Record X: immunoblotting]

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DataCite Commons2020-09-04 更新2024-07-25 收录
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https://figshare.com/articles/dataset/Genome-wide_chromatin_and_gene_expression_profiling_during_memory_formation_and_maintenance_in_adult_mice_Data_Record_X_immunoblotting_/3427418/1
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<b>Description</b><br>The file contains the immunoblotting results for chromatin markers H3K9ac, H3K4me3, H3K27ac, H3K27me3 and H4K12ac for tissues ACC and CA1, and one immunoblotting result for CA1 neuronal H3K9ac for conditions Naive (N), context at 1h (C) and context-shock at 1h (CS). Each table contains the tested sample names (based on their condition), chromatin mark, immunoblot channel, number ID and signal strength, total, area and background. Each replicate had its signals compared between the chromatin mark and H4 signal immunoblotting, resulting in the ratio signal and standard deviation between the signals. The average signal for each condition N, C and CS and standard deviation of said signals were also recorded, and finally, some immunoblots run twice for the same chromatin mark had their averages and standard deviations calculated together.<br>

<b>数据集描述</b><br>本数据集包含脑组织ACC与CA1的染色质标志物(chromatin marker)H3K9ac、H3K4me3、H3K27ac、H3K27me3及H4K12ac的免疫印迹(immunoblotting)结果,同时包含CA1神经元H3K9ac在未处理(Naive,记为N)、1小时情境暴露(记为C)及1小时情境-电击(记为CS)三种实验条件下的免疫印迹结果。<br>每张数据表均包含待测样本名称(基于其所属实验条件)、染色质修饰靶点、免疫印迹通道、编号ID以及信号总强度、信号面积与背景强度。<br>每一组生物学重复均会将目标染色质修饰的信号与H4组蛋白的免疫印迹信号进行比对,计算得到信号比值及其标准差。<br>同时记录了N、C、CS三种实验条件下的平均信号强度及其标准差;最终,针对同一染色质修饰靶点重复两次的免疫印迹实验,也会合并计算其平均信号与标准差。
提供机构:
figshare
创建时间:
2016-06-10
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