Characterization of three Epithelial Ovarian Cancer cell lines resistant to cisplatin (microRNA).

Epithelial ovarian cancer (EOC) represents the gynecologic cancer with the highest mortality rate. Despite a high initial response to chemotherapy, the majority of patients with advanced EOC relapses and subsequently develops chemoresistance that results in treatment failure and death of the patient. AIM: we have generated an isogenic model of PT-resistance in a panel of 3 EOC cell lines. microRNA profiling was used to identify new molecular pathway that regulate chemoresistance. microRNA experiments were performed in 3 Epithelian Ovarian Cancer cell lines Platinum-resistant vs their parental, with Agilent platform according to Agilent protocol for microRNA profile. Bioinformatics analyses were performed by Gene Springs上皮性卵巢癌（Epithelial ovarian cancer, EOC）是死亡率最高的妇科恶性肿瘤。尽管晚期上皮性卵巢癌患者初始对化疗的应答率较高，但多数患者会出现疾病复发，继而产生化疗耐药，最终导致治疗失败与患者死亡。研究目的：本研究构建了一组包含3株上皮性卵巢癌细胞系的铂类耐药同基因模型。采用微小RNA（microRNA）表达谱分析，筛选调控化疗耐药的全新分子通路。本研究以3株铂类耐药上皮性卵巢癌细胞系及其亲本对照细胞为实验对象，采用安捷伦（Agilent）平台并严格遵循其官方微小RNA表达谱分析实验流程完成全部微小RNA相关实验。生物信息学分析由Gene Springs平台完成。