Whole genome-based antimicrobial resistance and virulence profiling of Staphylococcus aureus isolates from chronic leg ulcer patients in Kilimanjaro Tanzania

Chronic leg ulcers are hard to treat and can be a burden, particularly in resource-limited settings where diagnosis is a challenge. Staphylococcus aureus is among the common bacteria isolated from chronic wounds with a great impact on wound healing, particularly in patients with co-morbidities. In this study, whole genome sequencing was used to assess antimicrobial resistance and virulence to support therapeutic choices for physicians, and importantly curb the development and spread of antibiotic resistance in the study locality.

慢性腿部溃疡治疗难度较高，会给患者带来沉重负担，在医疗资源匮乏地区尤为如此，此类地区的溃疡诊断本身亦颇具挑战。金黄色葡萄球菌（Staphylococcus aureus）是慢性伤口分离培养中常见的病原菌之一，对伤口愈合影响显著，在合并基础疾病的患者群体中更为突出。本研究采用全基因组测序技术，评估病原菌的抗菌药物耐药性与毒力特征，旨在为临床医师的治疗决策提供依据，同时重点遏制本研究区域内抗生素耐药性的产生与传播。