Schematic diagram of full length and truncated MPS-1.

(A) Various MPS-1 constructs with MPS-1(1–256), MPS-1(74–256) and MPS-1(134–256) depicted from top to bottom. The black segment (residues 46 to 68) represents the predicted transmembrane helix; (B) Model of the MPS1 domains showing the two phosphorylation sites within the cytoplasmic C-terminal domain. (C) SDS-PAGE of the purified MPS-1 proteins. Lane 1: molecular weight marker; lanes 2-4: purified MPS-1(1–256), MPS-1(74–256) and MPS-1(134–256).(A) 从上至下依次呈现三种MPS-1重组构建体：MPS-1(1–256)、MPS-1(74–256)与MPS-1(134–256)。其中黑色区段（残基46至68）为预测的跨膜螺旋（transmembrane helix）； (B) MPS-1结构域模型，展示了细胞质C端结构域内的两处磷酸化位点； (C) 纯化MPS-1蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）结果：泳道1为分子量标准参照物；泳道2至4分别对应纯化的MPS-1(1–256)、MPS-1(74–256)及MPS-1(134–256)。